2015/12/24 上午10:24:28 星期四
特别鸣谢86361班校友捐赠制作环境与生物工程学院网站
师资队伍

孔金明

孔金明
教授,博士生导师

办公电话:025-84303109   

电子邮件:j.kong@njust.edu.cn


教育背景:

1998920018月,新加坡南洋理工大学,化学,博士;

1988919921月,北京医科大学,药物化学,硕士;

1981919856月,郑州大学,物理化学,学士。


研究经历:

20119现在,南京理工大学,环境与生物工程学院,教授;

2009820107月,英国南安普敦大学,化学学院,博士后研究员;

2005720095月,新加坡微电子研究院,高级研究工程师;

2005320057月,国立新加坡大学,土木工程学院,博士后研究员;

2003620052月,新加坡南洋理工大学,机械制造工程学院,博士后研究员;

20021120033月,国立新加坡大学,化工学院,博士后研究员;

1992219982月,北京日用化学研究所,研究工程师;

1985919889月,河南省化工学校,教师。


科研情况简介:

从事多种基因、蛋白质和微流体生物芯片的研制。包括DNA芯片,登革热诊断芯片,心肌蛋白诊断芯片,硅纳米导线的多重免疫分析生物芯片开发,全自动化的基因测序芯片等。迄今为止,负责主持多项国家级研究项目的选题、申请、实施和总结,其中包括新加坡国家科技研究局基金2项,国家自然科学基金委项目2项,作为首席科学家主持国家高技术研究发展计划(863计划)基金1项等,总金额超过540万。并获得数项美国、新加坡和世界专利,正在申请多项中国专利。是国家卫计委“重大新药创制”科技重大专项和山东省自然科学基金重大基础研究项目特邀评审专家。

已在Journal of American Chemistry Society, Angewandte Chemie International Edition, Analytical Chemistry, Biosensors and Bioelectronics, Journal of Materials Chemistry B, Chemical Engineering Journal, Sensors and Actuators B: Chemical, ChemistryAn Asian Journal, Analytica Chimica Acta, Applied Physics Letters, Analyst, Talanta, Analytical Methods, Microchimica Acta等国际著名期刊上发表论文60篇,最高单篇文章他引已经超过746次,自2003 发表至今,已经连续14 年被该杂志选为TOP 25 的文章,10 次位列第一。另有一篇文章被国际顶级期刊《柳叶刀》(Lancet)引用。论文总引用率超过2501 次。有着多年的海外科学研究经验,跟许多国际和国内研究机构有着良好的合作关系。为联合培养高水平的博硕士研究人才创造了条件。是大学里上课最受欢迎的50位老师之一,培养的学生中已经有1位博士研究生和2位硕士生获得国家奖学金,1位硕士生获得南京理工大学优秀硕士学位论文。


主要研究方向:

化学和生物传感器,应用于光学/电学生物传感器的纳米材料和纳米技术研究,表面和界面的生物功能化,微分析系统生物芯片研究。


科研项目:

一、作为项目负责人的基金:

  (1) 新加坡科技研究局项目

[1] Nucleic Acids Electrical Sensing by Using Phosphate Backbond-Pectin Templated Silver Nanowire (起止时间: 2007720087, 项目经费: 25万新元);

[2] Ultrasensitive Electrical Detection of Nucleic Acids by Hematin Catalysed Silver Nanoparticle Formation in Nanogap Biosensor (起止时间: 2007720087, 项目经费: 20万新元)

(2) 中国科学基金项目

[1] 国家自然科学基金面上项目《基于肽核酸入侵的微电极阵列高灵敏双链DNA直接测定研究》(项目批准号: 21575066, 起止时间:2016.012019.12, 项目经费:76.64万);

[2] 国家自然科学基金主任基金项目《叉指微电级器件上的核酸分子超灵敏电检测研究》(项目批准号: 21345002, 起止时间: 20141201412, 项目经费: 10万, 已结题);

[3] 国家高技术研究发展计划(863计划)《基因工程菌高密度发酵表达胶原蛋白及高效分离工艺的关键技术及其产业化》(起止时间: 20141201612, 项目经费: 253万, 已结题);

[4] 南京留学人员科技活动项目择优资助《新型超灵敏生物分子电化学检测及其在肿瘤早期诊断中的应用》(起止时间: 20131201312, 项目经费: 2万, 已结题);

[5] 南京理工大学科研启动费《多糖辅助的生物分子超灵敏电化学检测及其在肿瘤早期诊断中的应用》(起止时间: 201112201312, 项目经费: 10万, 已结题)


二、作为项目参加人的基金:

[1] 国家自然科学基金项目《哺乳类动物中血红素蛋白调控的硫化物新陈代谢机理》(起止时间: 20131201612, 项目经费: 80万, 已结题);

[2] 南京理工大学高等教育教学改革研究课题《工科《分子生物学》探究式学习课堂教学模式探索》(起止时间: 2012120136, 项目经费: 0.73万)


发表论文:

2017年:

[1] Hu, Q., Wang, Q., Sun, G., Kong, J*., Zhang, X*. Electrochemically Mediated Surface-Initiated de Novo Growth of Polymers for Amplified Electrochemical Detection of DNA. Analytical Chemistry, 2017, 89, 9253−9259

[2] Hu, Q., Li, L., Sun, G., Li, D., Kong, J*., Huang, W., Zhang, X*. 5-Carboxyfluorescein: intrinsic peroxidase-like catalytic activity and its application in the biomimetic synthesis of polyaniline nanoplatelets. Journal of Materials Chemistry B, 2017, 5, 59375941.

[3] Mei, Y., Hu, Q., Zhou, B., Zhang, Y., He, M., Xu, T., Li, F., Kong, J*. Fluorescence Quenching Based Alkaline Phosphatase Activity Detection. Talanta, 2017, 176, 5258.

[4] Liu, Q., Hu, Q., Li, L., Kong, J*., Zhang, X*. Click Chemistry-based Aptasensor for High Sensitive Electrochemical Detection of Thrombin. Analytical Methods, 2017, 9, 38253830.

[5] Hu, Q., Ma, K., Mei, Y., He, M., Kong, J*., Zhang, X*. Metal-to-ligand charge-transfer: Applications to visual detection of β-galactosidase activity and sandwich immunoassay. Talanta, 2017, 167, 253259.

[6] Hu, Q., Zhou, B., Dang, P., Li, L., Kong, J*., Zhang, X*. Facile colorimetric assay of alkaline phosphatase activity using Fe(II)-phenanthroline reporter. Analytica Chimica Acta, 2017, 950, 170177.

[7] Hu, Q., He, M., Mei, Y., Feng, W., Jing, S., Kong, J*., Zhang, X*. Sensitive and selective colorimetric assay of alkaline phosphatase activity with Cu(II)-phenanthroline complex. Talanta, 2017, 163, 146152.

[8] Hu, W., Yu, X., Hu, Q., Kong, J*., Li, L., Zhang, X*. Methyl Orange removal by a novel PEI-AuNPs-hemin nanocomposite. Journal of Environmental Sciences, 2017, 53, 278283.

[9] Gu, S., Ma, K., Kong, J*., Al-Ghanim, K. A., Mahboob, S., Liu, Y., Zhang, X*. Functionalized Polyethyleneimine-gold Nanoparticles-Porphyrin Nanocomposite for Electrochemical Glucose Biosensing. International Journal of Electrochemical Science, 2017, 12, 50925103.

[10] Feng, H., Gao, L., Ye, X., Wang, L., Xue, Z., Kong, J., Li, L*. Synthesis of a heptapeptide and its application in the detection of mercury(II) ion. Chemical Research in Chinese Universities, 2017, 33, 155159.


2016年:

[1] Hu, Q., Zhou, B., Li, F., Kong, J*., Zhang, X*. Turn-On Colorimetric Platform for Dual Activity Detection of Acid and Alkaline Phosphatase in Human Whole Blood. ChemistryAn Asian Journal, 2016, 11, 30403045.

[2] Hu, W., Zhang, J*., Kong, J*. Fluorescence Detection of DNA Based on Non-covalent π-π Stacking Interaction between 1-Pyrenebutanoic Acid and Hypericin. Analytical Sciences, 2016, 32, 523527.

[3] Hu, Q., Kong, J*., Li, Y., Zhang, X*. A signal-on electrochemical DNA biosensor based on potential-assisted Cu(I)-catalyzed azide-alkyne cycloaddition mediated labeling of hairpin-like oligonucleotide with electroactive probe. Talanta, 2016, 147, 516522.

[4] Feng, Z., Yu, J., Kong, J*., Wang, T*. A novel porous Al2O3 layer/AgNPs–Hemin composite for degradation of azo dyes under visible and UV irradiation. Chemical Engineering Journal, 2016, 294, 236245.

[5] Yan, M., Xie, H*., Zhang, Q., Qu, H., Shen, J., Kong, J. Hemin Based Biomimetic Oxidative Degradation of Acid Orange 7. Journal of Materials Science and Chemical Engineering, 2016, 4, 26−34.

[6] Wei, Q., Dong, J., Zhao, P., Li, M., Cheng, F., Kong, J., Li, L*. DNA binding, BSA interaction and SOD activity of two new nickel(II) complexes with glutamine Schiff base ligands. Journal of Photochemistry and Photobiology B: Biology, 2016, 161, 355367.


2015年:

[1] Hu, W., Boateng, D., Kong, J*., Zhang, X*. Advancement of Fluorescent Methods for Detection of Nitric Oxide. Austin Journal of Biosensors and Bioelectronics, 2015, 1, 1003.

[2] Hu, W., Ning, Y., Kong, J*., Zhang, X*. Formation of copper nanoparticles on poly(thymine) through surface-initiated enzymatic polymerization and its application for DNA detection. Analyst, 2015, 140, 56785684.

[3] Kong, J*., Yu, X., Hu, W., Hu, Q., Shui, S., Li, L., Han, X., Xie, H., Zhang, X*., Wang, T*. A biomimetic enzyme modified electrode for H2O2 highly sensitive detection. Analyst, 2015, 140, 77927798.

[4] Hu, W., Ning, Y., Kong, J*., Zhang, X*. Highly Sensitive Detection of Sequence-specific DNA with Morpholino-functionalized Magnetic Microspheres. Analytical Methods, 2015, 7, 67126717.

[5] Hu, W., Fu, G., Kong, J*., Zhou, S., Scafa, N., Zhang, X*. Advantages of Morpholino in the Development of Nucleic Acid Biosensors. American Journal of Biomedical Sciences, 2015, 7, 4051.

[6] Hu, W., Hu, Q., Li, L., Kong, J*., Zhang, X*. Detection of sequence-specific DNA with a morpholino-functionalized silicon chip. Analytical Methods, 2015, 7, 24062412.

[7] Hu, Q., Deng, X., Kong, J*., Dong, Y., Liu, Q., Zhang, X*. Simple and fast electrochemical detection of sequence-specific DNA via click chemistry-mediated labeling of hairpin DNA probes with ethynylferrocene. Analyst, 2015, 140, 41544161.

[8] Hu, Q., Hu, W., Kong, J*., Zhang, X*. Ultrasensitive electrochemical DNA biosensor by exploiting hematin as efficient biomimetic catalyst toward in situ metallization. Biosensors and Bioelectronics, 2015, 63, 269275.

[9] Hu, Q., Deng, X., Yu, X., Kong, J*., Zhang, X*. One-step conjugation of aminoferrocene to phosphate groups as electroactive probes for electrochemical detection of sequence-specific DNA. Biosensors and Bioelectronics, 2015, 65, 7177.

[10] Hu, Q., Hu, W., Kong, J*., Zhang, X*. PNA-based DNA assay with attomolar detection limit based on polygalacturonic acid mediated in-situ deposition of metallic silver on a gold electrode. Microchimica Acta, 2015, 182, 427434.


2014年:

[1] Yu, X., Kong, J*., Han, X., Zhang, X*. Combination of Hematin and PEDOT via 1-Pyrenebutanoic acid: A New Platform for Direct Electrochemistry of Hematin and Biosensing Applications. RSC Advance, 2014, 4, 4698046986.

[2] 于雪花孔金明*, 李连之张学记*. 基于聚3,4-乙撑二氧噻吩和1-芘丁酸构建的新型铁卟啉仿生传感器及其对邻苯二酚的催化研究. 分析化学, 2014, 42, 14001407.

[3] 胡佳慧, 侯万丰, 孔金明*, 张健*. 金丝桃素药理研究进展. 药物生物技术, 2014, 04, 377380.

[4] 李大力单丹孔金明*, 石若夫. 探究式教学模式下生物工程专业综合实验成绩评价体系的构建. 高校生物学教学研究(电子版), 2014, 4, 2325. (高校生物学教学研究(电子版) 20142015年度优秀论文).


2013年及以前:

[1] 李大力杨成丽孔金明*, 单丹. 网络环境中探究式分子生物学课程教学模式的构建. 大学教育, 2013, 19, 6365.

[2] Huang, L., Li, L*., Li, H., Tian, S., Cui, H., Kong, J*. Interaction Between Neuroglobin and Caffeine by Multispectroscopic Methods. Spectroscopy Letters, 2013, 46, 433440.

[3] Bian, L., Li, L*., Zhang, Q., Dong, J., Xu, T., Li, J., Kong, J*. Synthesis, crystal structures, DNA binding and cleavage studies of two oxovanadium (IV) complexes of 1,10-phenanthroline and Schiff bases derived from tryptophan. Transition Metal Chemistry, 2012, 37, 783790.

[4] Li, Y., Li, L*., Pu, X., Ma, G., Wang, E., Kong, J., Liu, Z., Liu, Y. Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd2+. Bioorganic & Medicinal Chemistry Letters, 2012, 22, 40144017.

[5] Yang, B*., Kong, J. Silicon-based Interdigitated Electrode Array for Highly Sensitive Heavy Metal Ion Sensing. Chemistry Letters, 2009, 38, 10721073.

[6] Kong, J*., Ferhan, A. R., Chen, X., Zhang, L., Balasubramanian, N. Polysaccharide templated silver nanowire for ultrasensitive electrical detection of nucleic acids. Analytical Chemistry, 2008, 80, 72137217.

[7] Fang, C*., Fan, Y., Kong, J., Gao, Z., Balasubramanian, N. Electrical detection of oligonucleotide using an aggregate of gold nanoparticles as a conductive tag. Analytical Chemistry, 2008, 80, 93879394.

[8] Kong, J. M*., Zhang, H., Chen, X. T., Balasubramanian, N., Kwong, D. L. Ultrasensitive electrical detection of nucleic acids by hematin catalysed silver nanoparticle formation in sub-microgapped biosensors. Biosensors and Bioelectronics, 2008, 24, 787791.

[9] Kong, J. M*., Wong, C. V., Gao, Z. Q., Chen, X. T. Preparation of silver nanoparticles by microwave-hydrothermal technique. Synthesis and Reactivity in Inorganic, Metal-Organic and Nano-Metal Chemistry, 2008, 38, 186188.

[10] Fang, C*., Fan, Y., Kong, J. M., Gao, Z. Q., Balasubramanian, N. Preparation of nanochain and nanosphere by self-assembly of gold nanoparticles. Applied Physics Letters, 2008, 92, 263108.

[11] Fan, Y., Chen, X., Kong, J., Tung, C. H., Gao, Z*. Direct detection of nucleic acids by tagging phosphates on their backbones with conductive nanoparticles. Angewandte Chemie International Edition, 2007, 46, 20512054.

[12] Yi Fan, Xiantong Chen, Chih-hang Tung, Jinming Kong, Zhiqiang Gao*. Detection of MicroRNAs using Target-guided Formation of Conducting Polymer Nanowires in Nanogaps. Journal of American Chemical Society, 2007, 129, 54375443.

[13] Zhiqiang Gao*, Ajay Agarwal, Alastair D. Trigg, Navab Singh, Cheng Fang, Chih-Hang Tung, Yi Fan, Kavitha D. Buddharaju, Jinming Kong. Silicon Nanowire Arrays for Label-Free Detection of DNA. Analytical Chemistry, 2007, 79, 32913297.

[14] Fang, C*., Fan, Y., Kong, J. M., Zhang, G. J., Linn, L., Rafeah, S. DNA-templated preparation of palladium nanoparticles and their application. Sensors and Actuators B: Chemical, 2007, 126, 684690.

[15] Liu, Y. H*., Kong, J. M., Chia, L. S., Goh, N. K. The Synergistic Effect of Traditional Chinese Medicine. Asian Journal of Chemistry, 2007, 19, 867882.

[16] Kong, J*., Li, L., Chia, L., Goh, N. Antioxidant capacity measurement of flavonoids by chemiluminescence method. Natural Product Research and Development, 2004, 16, 4649.

[17] Kong , J. M*., Tan, K. S., Goh, N. K., Chia, L. S. Traditional Chinese Medicine. Asia Pacific Biotechnology, 2004, 8, 790797.

[18] Kong, J*., Chen, R., Li, J., Xie, L., Wu, W. Comparative Study of CE and HPLC on the Separation of Flavonoids. Natural Product Research and Development, 2003, 16, 451457.

[19] Kong, J. M., Chia, L. S*., Goh, N. K., Chia, T. F., Brouillard, R. Analysis and biological activities of anthocyanins. Phytochemistry, 2003, 64, 923933. (http://top25.sciencedirect.com/subject/chemistry/6/journal/phytochemistry/00319422/archive/66/, from 2003 to now for consecutive 14 years, and 10 times ranked the first.)

[20] Kong, J. M., Goh, N. K., Chia, L. S*., Chia, T. F. Recent advances in traditional plant drugs and orchids. Acta Pharmacologica Sinica, 2003, 24, 721.

[21] Willams, C. A*., Greenham, J., Harborne, J. B., Kong, J. M., Goh, N. K., Chia, L. S., Saito, N., Toki, K., Tatsuzawa, F. Acylated Anthocyanins and Flavonol from Purple Flowers of Dendrobium cv. ‘Pompadour’. Biochemical Systematics and Ecology, 2002, 30, 667675.


发明专利:

[1] Kong, J., Fan, Y., Chen, X. Method of electrically detecting a nucleic acid molecule. (2010/10/11, US 20100285601, 美国专利).

[2] Kong, J., Fan, Y., Chen, X. Method of electrically detecting a nucleic acid molecule. (2010/06/15, SG 201002059-2, 新加坡专利).

[3] Kong, J., Fan, Y., Chen, X. Method of electrically detecting a nucleic acid molecule. (2009/04/02, WO 2009/041917, 世界专利).

[4] Gao, Z., Fan, Y., Chen, X., Kong, J. Method of electrically detecting a biological analyte molecule. (WO/2008/020813, 世界专利).

[5] Gao, Z., Fan, Y., Chen, X., Kong, J. Electrical detection of nucleic acids using target-guided formation of conducting polymer naowires in a Nanogap. (WO 2008/097190, 世界专利).

[6] Gao, Z., Fan, Y., Chen, X., Kong, J. Method of electrically detecting a biological analyte molecule. (US/2010/0194409, 美国专利).